Inhibition of the integrin signal constitutes a mouse iPS cell niche

In this study, we used three murine iPS cell lines, iPS‐MEF‐Ng‐20D‐17, iPS‐MEF‐Ng‐178B‐5 and iPS‐MEF‐Fb/Ng‐440A‐3, which were generated by different reprogramming methods. In general, these cell lines commonly need the feeder cells as a niche to culture. Recently, the effect of substrate stiffness is known in stem cell study. First, we focused on the mechanical properties of feeder cells, and then we speculated that feeder‐less culture might be made possible by using molecules in place of the mechanical properties of the niche. Finally, we found that the combination of disintegrin (echistatin) and 2i (GSK3 inhibitor and MEK inhibitor) is a sufficient condition for three murine iPS culture. This novel method of mimicking the murine iPS cell niche may be useful to understand signaling pathways to maintain the pluripotency of stem cells. We found that the combination of disintegrin (echistatin) and 2i (GSK3 inhibitor and MEK inhibitor) is a sufficient condition for mouse iPSC feeder‐less culture. This novel method of mimicking the miPS cell niche may be useful to understand signaling pathways to maintain the pluripotency of stem cells (Adapted from Hynes R. O. 2002.).
Source: Development, Growth and Differentiation - Category: Research Authors: Tags: Original Article Source Type: research