Engineered Riboswitch as a Gene-Regulatory Platform for Reducing Antibiotic Resistance

Antibiotic resistance (AR), the ability of a microorganism to withstand the effects of antibiotics, is a growing and increasingly serious global public health problem. Enzymatic activation of antibiotics though the production of β-lactamase is one of the main mechanisms causing AR. Synthetic riboswitch containing aptazyme is constructed in E. coli to regulate the expression of β-lactamase through small molecule–aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.
Source: Springer protocols feed by Biochemistry - Category: Biochemistry Source Type: news