Standardization and quality management in next-generation sequencing

Publication date: Available online 1 July 2016 Source:Applied & Translational Genomics Author(s): Christoph Endrullat, Jörn Glökler, Philipp Franke, Marcus Frohme DNA sequencing continues to evolve quickly even after >30years. Many new platforms suddenly appeared and former established systems have vanished in almost the same manner. Since establishment of next-generation sequencing devices, this progress gains momentum due to the continually growing demand for higher throughput, lower costs and better quality of data. In consequence of this rapid development, standardized procedures and data formats as well as comprehensive quality management considerations are still scarce. Here, we listed and summarized current standardization efforts and quality management initiatives from companies, organizations and societies in form of published studies and ongoing projects. These comprise on the one hand quality documentation issues like technical notes, accreditation checklists and guidelines for validation of sequencing workflows. On the other hand, general standard proposals and quality metrics are developed and applied to the sequencing workflow steps with the main focus on upstream processes. Finally, certain standard developments for downstream pipeline data handling, processing and storage are discussed in brief. These standardization approaches represent a first basis for continuing work in order to prospectively implement next-generation sequencin...

Source: Applied and Translational Genomics - July 15, 2016 Category: Genetics & Stem Cells Source Type: research