Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR)

Electron paramagnetic resonance (EPR) is an invaluable tool when studying systems with paramagnetic centers. It is a sensitive spectroscopic method, which can be used with dilute samples in aqueous buffer solutions. Here, we describe the basic procedure for recording an X-band EPR spectrum of a metalloprotein sample at low temperature. We also discuss basic optimization techniques to provide spectra with a high signal to noise ratio and minimum distortion.

Source: Springer protocols feed by Protein Science - March 27, 2014 Category: Biochemistry Source Type: news