The regulatory role of nickel on H3K27 demethylase JMJD3 in kidney cancer cells

In this study, kidney cancer cells namely human embryonic kidney 293-containing SV40 large T-antigen (HEK293T) and 786-0 were incubated with various concentrations of nickel chloride for 24 h before analysing the expression of three histone H3K27 methylation-modifying enzymes and H3K27me3 using quantitative real-time polymerase chain reaction, Western blot and immunofluorescence analyses. Our results showed that incubation of nickel chloride upregulated the expression of H3K27me3 demethylase jumonji domain-containing protein 3 (JMJD3) in kidney cancer cells, which was accompanied by the reduction in the protein level of H3K27me3. Enhanced demethylation of H3K27me3 may represent a novel mechanism underlying the carcinogenicity of nickel compounds.

Source: Toxicology and Industrial Health - June 26, 2016 Category: Toxicology Authors: Guo, X., Zhang, Y., Zhang, Q., Fa, P., Gui, Y., Gao, G., Cai, Z. Tags: Articles Source Type: research