Evaluation of macroscopic changes and the efficiency of DNA profiling from burnt teeth

Identification of human remains subjected to incineration is extremely challenging. Our study evaluates the macroscopic changes and efficiency of DNA profiling in burnt teeth under controlled temperature and time conditions. 28 teeth were exposed to temperatures between 100 and 700°C for a duration of 1–15min. Two non-burnt teeth were used as control. Macroscopic changes were evaluated and recorded. DNA was extracted using a silica-based methodology. Efficiency of DNA profiling was assessed through Quantitative PCR for STRs.
Source: Science and Justice - Category: Forensic Medicine Authors: Tags: Technical note Source Type: research