Association between interleukin-4 (IL-4), gene polymorphisms (C-589T, T+2979G, and C-33T) and migraine susceptibility in Iranian population: A case–control study

Publication date: Available online 10 June 2016 Source:Egyptian Journal of Medical Human Genetics Author(s): Nourollah Ramroodi, Mohammad Reza Javan, Nima Sanadgol, Mehdi Jahantigh, Tooba Nakhzari Khodakheir, Nasrin Ranjbar Background Migraine is a chronic neurological disease characterized by recurrent moderate to severe headaches commonly in association with neuro-inflammation. Interleukin-4 (IL-4), an anti-inflammatory cytokine, plays an important role in modulating pain threshold and has an essential role in stimulation of pain receptors in the trigeminal nerve fibers. Aim of the study The current study aimed to investigate the possible associations between IL-4 single nucleotide polymorphisms (SNPs) and susceptibility to migraine in Iranian patients. Patients and methods In a prospective case–control study, we studied blood samples of 190 patients with migraine (migraineurs) and 200 healthy controls (HCs) for analysis of gene variants. Genotyping for the IL-4 SNPs: C-589T (rs2243250), T+2979G (rs2227284), and C-33T (rs2070874) were performed using PCR-RFLP. Statistical analysis was performed using the SPSS version 21.0 (SPSS, Chicago) and SNPStats version 1.14.0. Results and conclusion Among IL-4 SNPs, rs2243250 (TC genotype, OR=0.25, 95% CI=0.13–0.50, P =0.001) and rs2227284 (TG and TT genotypes, OR=0.44, 95% CI=0.23–0.92, P =0.029 and OR=0.38, 95% CI=0.18–0.79, P =0.009 respectively) were significantly associated with migraine. No signific...
Source: Egyptian Journal of Medical Human Genetics - Category: Genetics & Stem Cells Source Type: research