Effect of hemicellulolytic enzymes to improve sugarcane bagasse saccharification and xylooligosaccharides production

In this study, only two hemicelulases, the endo-1,4-xylanases (GH11) from Penicillium funiculosum (XynC11/CAC15487) and the feruloyl esterase (CE1) from Clostridium thermocellum (CtFAE/ATCC27405), effectively broke-down hemicellulose from pretreated sugarcane bagasse (up to 65%), along with the production of xylooligosaccharides (XOS). Our results also demonstrated that GH11 and CE1 can improve biomass saccharification by cellulases. Treatment with these two enzymes followed by a commercial cellulase cocktail (Accellerase®1500) increased saccharification of pretreated lignocellulose by 24%. Collectively, our data contributes to the rational design of more efficient and less expensive enzyme mixtures, targeting the viable production of bioethanol and other biorefinery products. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - June 6, 2016 Category: Biochemistry Source Type: research