Biochemical Properties of a New Nitrile Reductase Cloned from Pectobacterium carotovorum

Publication date: Available online 4 June 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Min Li, Zheng Zhou, Zhi-Jun Zhang, Hui-Lei Yu, Jian-He Xu Nitrile reductase is a newly discovered enzyme class that can catalyze the reduction of nitriles directly to amines, a very important reaction in synthetic organic chemistry. However, little progress has been made towards the application of this biocatalysis reaction for organic synthesis since the initial discovery of nitrile reductase for over ten years. One of the potential reasons may be due to the lack of structural information and biochemical properties, in addition to its inherent narrow substrate scope. Meanwhile, the highly conserved active site residues make it a great challenge to widen the substrate spectrum of nitrile reductase by means of protein engineering approaches. To explore more nitrile reductases with different characteristics (e.g. specific activity, thermostability, etc) for potential synthetic applications, a new nitrile reductase, designated as PcNRed, was discovered from the genome of Pectobacterium carotovorum, with a moderate specific activity of 366U/g protein towards its natural substrate, preQ0. The PcNRed was subsequently overexpressed in Escherichia coli BL21 (DE3), purified to homogeneity and then its biochemical properties were examined. The optimal reaction temperature and pH were determined to be 40°C and 7.4, respectively. The thermal deactivation process of ...
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research