An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1

Publication date: June 2016 Source:Biomolecular Detection and Quantification, Volume 8 Author(s): Alison S. Devonshire, Rebecca Sanders, Alexandra S. Whale, Gavin J. Nixon, Simon Cowen, Stephen L.R. Ellison, Helen Parkes, P. Scott Pine, Marc Salit, Jennifer McDaniel, Sarah Munro, Steve Lund, Satoko Matsukura, Yuji Sekiguchi, Mamoru Kawaharasaki, José Mauro Granjeiro, Priscila Falagan-Lotsch, Antonio Marcos Saraiva, Paulo Couto, Inchul Yang, Hyerim Kwon, Sang-Ryoul Park, Tina Demšar, Jana Žel, Andrej Blejec, Mojca Milavec, Lianhua Dong, Ling Zhang, Zhiwei Sui, Jing Wang, Duangkamol Viroonudomphol, Chaiwat Prawettongsopon, Lina Partis, Anna Baoutina, Kerry Emslie, Akiko Takatsu, Sema Akyurek, Muslum Akgoz, Maxim Vonsky, L.A. Konopelko, Edna Matus Cundapi, Melina Pérez Urquiza, Jim F. Huggett, Carole A. Foy Measurement of RNA can be used to study and monitor a range of infectious and non-communicable diseases, with profiling of multiple gene expression mRNA transcripts being increasingly applied to cancer stratification and prognosis. An international comparison study (Consultative Committee for Amount of Substance (CCQM)-P103.1) was performed in order to evaluate the comparability of measurements of RNA copy number ratio for multiple gene targets between two samples. Six exogenous synthetic targets comprising of External RNA Control Consortium (ERCC) standards were measured alongside...
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research