A/T gap tolerance in the core sequence and flanking sequence requirements of non-canonical p53 response elements

In this study, we investigated the possible number of A/T bases used by p53 and showed that a six-base A/T gap CATATATG core sequence was the maximum A/T gap in the p53 response element that could be upregulated by p53 and p63. Canonical and non-canonical p53 response elements also have three-base flanking sequences. A/T bases could be substituted by G/C bases, including CACACG and CGTGTG, but not CGCGCG. We found that the SV40 promoter with functional six- and two-base A/T gap core sequences could be activated by TAp63 and that TAp63 could upregulate SV40 small and large T antigens expression in COS7 cells. We also found that the distal region of PUMA promoter with functional two six-base A/T gap core sequences could be activated by TAp63 in 293T cells. These new findings could provide novel rules for the non-canonical p53 family response element and could extend the entire p53 family regulation network.

Source: Journal of Biochemistry - May 30, 2016 Category: Biochemistry Authors: Cai, B.-H., Chao, C.-F., Lin, H.-C., Huang, H.-Y., Kannagi, R., Chen, J.-Y. Tags: Regular Papers Source Type: research