The use of the lymphocyte cytokinesis-block micronucleus assay for monitoring pesticide-exposed populations

Publication date: Available online 26 May 2016 Source:Mutation Research/Reviews in Mutation Research Author(s): Claudia Bolognesi, Nina Holland Pesticides are widely used around the world, and hundreds of millions of people are exposed annually in occupational and environmental settings. Numerous studies have demonstrated relationships between pesticide exposure and increased risk of cancers, neurodegenerative and neurodevelopmental disorders, respiratory diseases and diabetes. Assessment of genotoxicity of pesticides and biomonitoring their effects in exposed populations is critical for a better regulation and protection, but it can be complicated because pesticides are often used as complex mixtures. The cytokinesis-block micronucleus assay in human lymphocytes (L-CBMN) is a validated method of assessment of DNA damage induced by clastogenic and aneuploidogenic mechanisms. The goal of this review is to provide an updated summary of publications on biomonitoring studies using this assay in people exposed to pesticides in different settings, and to identify gaps in knowledge, and future directions. A literature search was conducted through MedLine/PubMed and TOXLINE electronic databases up to December 2015. A total of 55 full-text articles, related to 49 studies, excluding reviews, were selected for in depth analysis, divided by the settings where exposures occurred, such as chemical plant workers, pesticide sprayers, floriculturists, agricultural workers and non-occu...
Source: Mutation Research Reviews in Mutation Research - Category: Genetics & Stem Cells Source Type: research