Genome engineering: Presenting Argonaute, the genome editor

Nature Reviews Molecular Cell Biology 17, 332 (2016). doi:10.1038/nrm.2016.68 Author: Eytan Zlotorynski Argonaute proteins are endonucleases that typically require 5′-phosphorylated, short single-stranded guide RNAs (gRNA) for targeting and cleavage of transcripts; however, some prokaryotic Argonautes cleave DNA, similarly to the endonuclease Cas9. Unlike Cas9, DNA-cleaving Argonautes use gDNA rather than gRNA and do not require auxiliary sequences
Source: Nature Reviews Molecular Cell Biology - Category: Cytology Authors: Tags: Research Highlight Source Type: research