A transcriptional co-factor YAP regulates IFNT expression via transcription factor TEAD in bovine conceptuses

Interferon tau (IFNT) is the pregnancy recognition protein in all ruminants and its expression is restricted to trophoblast cells. IFNT production increases as the conceptus elongates, however, its expression is down-regulated soon after the initiation of conceptus attachment to the uterine epithelium. Our previous study identified that among eight bovine IFNT genes, only two forms of IFNTs, IFNT2 and IFN-tau-c1, were expressed by the conceptuses during the peri-attachment period. To characterize whether Hippo signaling including a transcription co-factor Yes associated protein (YAP) was involved in the IFNT regulation, we examined the expression and effects of YAP and/or TEAD in human choriocarcinoma JEG3 and bovine trophoblast CT-1 cells, and in bovine conceptuses obtained from day 17, 20 or 22 pregnant animals (pregnant day 19.5 = day of conceptus attachment to the endometrium).
Source: Domestic Animal Endocrinology - Category: Endocrinology Authors: Source Type: research