Determination of trace amounts of Brown HT as a food dye by CPE-Scanometry method

In this study, trace amounts of Brown HT was extracted, preconcentrated and determined by cloud point extraction-scanometry (CPE-Scanometry) as a simple, fast and inexpensive method. The procedure is based on CPE of Brown HT from aqueous solution, diluting the extracted surfactant-rich phase with ethanol to 500μL, transfering of proper volume of it to Plexiglas® cell and scanning of cells. Then the color of each cell was analyzed by the software written in visual basic (VB 6) media to red, green and blue values. Effective parameters on the extraction efficiency such as pH of the system, the concentration of the surfactant, equilibration temperature and time, were investigated and optimized. Under the optimal conditions, the calibration curve was linear in the range of 0.06-2.60mgL−1. The detection limit of the proposed method was 0.04mgL−1. In addition, the effect of some foreign species including cations, anions and dyes were investigated. The method was applied successfully for determination of Brown HT in some water samples.

Source: Journal of Taibah University for Science - May 13, 2016 Category: Science Source Type: research