Fish oil-derived n-3 polyunsaturated fatty acids reduce NLRP3 inflammasome activity and obesity-related inflammatory cross-talk between adipocytes and CD11b+ macrophages

Adipocyte-macrophage cross-talk propagates immune responses in obese adipose tissue (AT). Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) mitigate inflammation, partly through up-regulation of adiponectin; however, specific mechanisms are unclear. We determined if adipocyte-macrophage cross-talk could be mitigated by dietary LC n-3 PUFA, and if this was dependent on adiponectin-mediated signaling. We utilized an in vitro co-culture model mimicking the ratio of adipocytes:macrophages in obese AT, whereby 3 T3-L1 adipocytes were co-cultured with splenic CD11b+-macrophages from C57BL/6 mice fed high-fat control (HF-CON; 34% w/w fat) or fish-oil diets (HF-FO; 34% w/w fat containing 7.6% w/w FO), as well as mice fed low-fat control (LF-CON; 10% w/w fat) or FO diets (LF-FO; 10% w/w fat containing 3% w/w FO).

Source: The Journal of Nutritional Biochemistry - April 27, 2016 Category: Biochemistry Authors: Anna A. De Boer, Jennifer M. Monk, Danyelle M. Liddle, Amber L. Hutchinson, Krista A. Power, David W.L. Ma, Lindsay E. Robinson Source Type: research