Identification and characterization of a novel Old Yellow Enzyme from Bacillus subtilis str.168

Publication date: Available online 27 April 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Xiqian Sheng, Ming Yan, Lin Xu, Miao Wei We identified and characterized YqiG, a novel Old Yellow Enzyme (OYE) from Bacillus subtilis str.168, as a member of the “thermophilic-like” subfamily. It is most related to XenA from Pseudomonas putida, with 37.9% identity, but it exhibits certain differences in sequence and enzyme properties. The YqiG can reduce various activated alkenes and exhibits high temperature (60°C for 12h) and pH stability (pH 4.0 and 9.0 for 12h), which indicates that it has a great potential for biocatalysis. However, it shows low tolerance toward organic solvents. Also the YqiG shows high activities towards maleimide, dimethyl maleate and acyclic ketones (55.5U/mg, 6.22U/mg and 2.97U/mg, respectively). Besides, The YqiG can catalyze citral to (S)-citronellal with a low rate but high ee % (>99%). Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - April 27, 2016 Category: Biochemistry Source Type: research