Production of cinnamoyl lipids using immobilized Proteus vulgaris K80 lipase and an evaluation of their antioxidant activity

In this study, ethyl cinnmate (40mM) and triolein (240mM) were used as substrates and immobilized Proteus vulgaris K80 lipase (1U) was used as enzyme catalyst. When the interesterification reaction was performed in 5mL n-hexame/toluene (85:15, v/v) at 35°C for 72h, cinnamoyl monooleyl glycerol (CMOG) and cinnamoyl dioleyl glycerol (CDOG) were produced and the conversion yield was 70%. These cinnamoylated lipid products were collected by preparative liquid chromatography and identified using high resonance mass spectrometry. CMOG and CDOG showed radical scavenging activities of 45% and 69%, respectively, in n-hexane system using DPPH assay and 56% and 20%, respectively, in dichloromethane/DMSO (8:2, v/v) system using ABTS assay. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research