Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing

This study established effective clinically compatible strategies for cold (4°C) storage of hPSC-CMs cultured as two-dimensional (2D) monolayers and three-dimensional (3D) aggregates. Cell recovery of 2D monolayers of hPSC-CMs was found to be dependent on the time of storage, and 3D cell aggregates were more resistant to prolonged cold storage than 2D monolayers. Of note, it was demonstrated that 7 days of cold storage did not affect hPSC-CM ultrastructure, phenotype, or function. This study provides important insights into the cold preservation of PSC-CMs that could be valuable in improving global commercial distribution of hPSC-CMs.

Source: Stem Cells Translational Medicine - April 17, 2016 Category: Stem Cells Authors: Correia, C., Koshkin, A., Carido, M., Espinha, N., Šarić, T., Lima, P. A., Serra, M., Alves, P. M. Tags: Protocols and Manufacturing for Cell-Based Therapies, Cardiac Stem Cells Source Type: research