Semi‐synthesis of a tag‐free O‐GlcNAcylated tau protein by sequential chemoselective ligation

We present the first semi‐synthesis of the full‐length tau protein carrying an O‐GlcNAc moiety at S400, a site shown to inhibit tau phosphorylation at neighboring serine residues. Native chemical ligation was combined with expressed protein ligation to obtain the desired homogeneously modified tau protein. Pure protein was finally obtained by means of a photocleavable biotin tag.
Source: Journal of Peptide Science - Category: Biochemistry Authors: Tags: Special Issue Article Source Type: research