Transcript levels of genes implicated in steroidogenesis in the testes and fat tissue in relation with androstenone accumulation in fat of pubertal pigs

The present study was performed to measure mRNA levels of steroidogenic enzymes in testes and fat tissue and determine whether they are related to fat androstenone level. Real time PCR experiments were performed on 26 testes and 12 adipose tissue samples from pubertal boars using 21 genes. The absence of significant correlations between fat androstenone and the transcriptional activity of the SRD5A2 and SRD5A3 genes but the high correlation coefficient with that of the SRD5A1 gene (r = 0.62, P < 0.05) suggest that the enzyme coded by SRD5A1 is mainly responsible for the last step of androstenone synthesis.
Source: Domestic Animal Endocrinology - Category: Endocrinology Authors: Source Type: research