Quantitative Analysis of Total Plasma Homocysteine by LC-MS/MS.

Quantitative Analysis of Total Plasma Homocysteine by LC-MS/MS. Curr Protoc Hum Genet. 2016;89:17.21.1-17.21.10 Authors: Yuan L, Sharer JD Abstract Homocysteine is a nonessential, sulfur-containing amino acid involved in one-carbon (folate) metabolism. A number of inherited and acquired conditions cause increased accumulation of this metabolite in blood (homocysteinemia) and other biofluids. Homocysteinemia is a risk factor for cardiovascular disease, including recurrent thrombosis. Accurate measurement of total plasma homocysteine is an important element in the diagnostic evaluation of these disorders. While a number of different methods have been developed for this purpose, the focus of this unit will be on a specific technique utilizing liquid chromatography-tandem mass spectrometry, which provides several advantages in terms of speed, sensitivity, and specificity. © 2016 by John Wiley & Sons, Inc. PMID: 27037487 [PubMed - as supplied by publisher]
Source: Current Protocols in Human Genetics - Category: Genetics & Stem Cells Tags: Curr Protoc Hum Genet Source Type: research