Effect of deep eutectic solvent mixtures on lipase activity and stability

Publication date: Available online 30 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Sung Hee Kim, Saerom Park, Hyejeong Yu, Ji Hyun Kim, Hyung Joo Kim, Yung-Hun Yang, Yong Hwan Kim, Kwang Jin Kim, Eunsung Kan, Sang Hyun Lee DESs (deep eutectic solvents) have many potential applications as cosolvents or anhydrous reaction media for biocatalytic reactions, owing to their non-volatility, non-flammability, non-toxicity, biocompatibility, biodegradability, and low cost. In this work, choline chloride ([Ch]Cl)-based DESs and DES mixtures containing two hydrogen bond donors were used as cosolvents to enhance the activity and stability of Candida rugosa lipase in aqueous reactions. The activity of lipase in an aqueous solution of [Ch]Cl:urea:glycerol was 155% higher than that in buffer. The half-life time of lipase at 40°C in an aqueous solution of [Ch]Cl:glycerol was enhanced by 9.2 times. The lipase showed the highest acid stability and base stability in the aqueous solutions of [Ch]Cl:glycerol:thiourea and [Ch]Cl:ethylene glycol:formamide, respectively. In general, glycerol-containing DES mixtures were very useful in enhancing the activity and stability of lipase, while formamide-containing DES mixtures could not efficiently enhance the activity and stability of lipase. To understand the effect of DES mixtures on the activity and stability of lipase in aqueous solution, four solvatochromic parameters of DES mixtures were determin...
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research