Role of cyclic alkyl group in conformational instability of Tannase

Publication date: Available online 26 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Guangjun Nie, Rui Zhao, Wuyue Sun, Yu Gao, Xiangxiang Zhu, Zhiming Zheng, Wenjin Yue The conformational stability of enzyme has a crucial effect on its catalytic performance. The effects of six organic solvents with different structures on the conformational stability of tannase were studied using Fourier transform infrared spectroscopy in this work. This results indicated that the cyclic structure of organic solvent plays a negative role in the conformational stability of tannase. The alkyl group of organic solvent has an interaction with the groups of oxygen and nitrogen of tannase, and the interaction goes against the conformational stability. The findings potentially provide a deep insight into the relationship between the biocatalytic activity and conformational stability of enzymes and push the study on the interaction of enzyme with organic solvent. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - March 26, 2016 Category: Biochemistry Source Type: research