Characterization of the 5-flanking region of the human TP53 gene and its response to the natural compound, Resveratrol

In this study, we constructed a Luciferase expression vector, pGL4-TP53-551, containing 551 bp of the 5’-upstream region of the human TP53 gene, which was then transfected into HeLa S3 cells. A Luciferase assay showed that Rsv treatment increased the promoter activity of the TP53 gene in comparison to that of PIF1. Detailed deletion and mutation analyses revealed that Nkx-2.5 and E2F-binding elements are required in addition to duplicated GGAA (TTCC), for the regulation of TP53 promoter activity. In this study, it is suggested that the transient induction of TP53 gene expression by Rsv treatment might be partly involved in its anti-aging effect through maintenance of chromosomal DNAs.

Source: Journal of Biochemistry - March 20, 2016 Category: Biochemistry Authors: Uchiumi, F., Shoji, K., Sasaki, Y., Sasaki, M., Sasaki, Y., Oyama, T., Sugisawa, K., Tanuma, S.-I. Tags: Regular Papers Source Type: research