A High-Throughput Assay for the Detection of {alpha}-Dystroglycan N-Terminus in Human Uterine Fluid to Determine Uterine Receptivity

In this study, we first validated three monoclonal antibodies raised against α-DG-N in our system, and then established a sandwich ELISA suitable for the detection of α-DG-N in human uterine fluid. This ELISA detected significantly higher concentrations of α-DG-N in uterine fluid of women in the receptive phase. We believe this newly established α-DG-N ELISA may provide an important tool in the development of noninvasive strategies to detect uterine receptivity in women.
Source: Journal of Biomolecular Screening - Category: Molecular Biology Authors: Tags: Technical Notes Source Type: research