Dynamics of L cells along the crypt-villous axis in the chicken ileum

The dynamics of L cells along the crypt-villous axis were investigated in the ileum of male White Leghorn chicks (7 d of age, n = 5). Immunohistochemistry was used to detect the expression of glucagon-like peptide (GLP)-1 and an in situ hybridization technique to detect proglucagon messenger RNA (mRNA). Immunocytochemistry using colloidal gold was also applied to quantitatively evaluate the GLP-1 content. The cells expressing a proglucagon mRNA signal were distributed mainly in the crypts and the bottom of the villi but were never found in the upper part of the villi.

Source: Domestic Animal Endocrinology - March 2, 2016 Category: Endocrinology Authors: K. Nishimura, K. Hiramatsu, T. Watanabe Source Type: research