In-vitro Antioxidant and Antimicrobial Activity of Bougainvillea glabra Flower

The aim of the present study is to assess the phytochemical nature, antioxidant and antimicrobial activities of methanolic extract of Bougainvillea glabra flower. Antioxidants play an important role in protecting cellular damage by reactive oxygen species. The fractions of the flower extract were screened for antioxidant activities using DPPH radical scavenging activity, reducing power assay, total antioxidant capacity and reduction of ferric ions by o-phenanthroline color method. Antimicrobial activities of different solvent fractions were tested against gram positive and gram negative bacterial strains by observing the zone of inhibition using disc diffusion method, where eImipenem (10 μg disc–1) was used as the standard. The bacterial strains used in the study were Staphylococcus aureus , Bacillus cereus , Escherichia coli and Pseudomonas aeruginosa. The extracts revealed the presence of phytochemicals. Almost all three fractions exhibited remarkable antioxidant and antimicrobial activity in terms of all the assays tested. Water fraction showed DPPH radical scavenging activity with IC50 value of 135.73 μg mL–1. Not surprisingly, the n-hexane fraction showed excellent antioxidant activity for reduction of ferric ions by o-phenanthroline color method. All three types of fractions showed inhibitory effect against all tested bacteria except P. aeruginosa. These findings indicate compounds isolated from carbon tetrachloride and water fractions possess pharmacolo...
Source: Research Journal of Medicinal Plant - Category: Complementary Medicine Source Type: research