Development of NIST standard reference material 2373: Genomic DNA standards for HER2 measurements

Publication date: June 2016 Source:Biomolecular Detection and Quantification, Volume 8 Author(s): Hua-Jun He, Jamie L. Almeida, Steve P. Lund, Carolyn R. Steffen, Steve Choquette, Kenneth D. Cole NIST standard reference material (SRM) 2373 was developed to improve the measurements of the HER2 gene amplification in DNA samples. SRM 2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five components are derived from the human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474. The certified values are the ratios of the HER2 gene copy numbers to the copy numbers of selected reference genes DCK, EIF5B, RPS27A, and PMM1. The ratios were measured using quantitative polymerase chain reaction and digital PCR, methods that gave similar ratios. The five components of SRM 2373 have certified HER2 amplification ratios that range from 1.3 to 17.7. The stability and homogeneity of the reference materials were shown by repeated measurements over a period of several years. SRM 2373 is a well characterized genomic DNA reference material that can be used to improve the confidence of the measurements of HER2 gene copy number.

Source: Biomolecular Detection and Quantification - March 9, 2016 Category: Molecular Biology Source Type: research