Docosahexaenoic acid and palmitic acid reciprocally modulate monocyte activation in part through endoplasmic reticulum stress

Palmitic acid (C16:0) and TLR2 ligand induce, but docosahexaenoic acid (DHA) inhibits monocyte activation. C16:0 and TLR2 or TLR4 ligand induce certain ER stress markers; thus, we determined whether ER stress induced by these agonists is sufficient to induce monocyte activation, and whether the ER stress is inhibited by DHA which is known to inhibit C16:0- or ligand-induced TLR activation. Monocyte activation and ER stress were assessed by TLR/inflammasome-induced IL-1β production, and phosphorylation of IRE-1 and eIF2 and expression of CHOP, respectively in THP-1 cells.

Source: The Journal of Nutritional Biochemistry - March 8, 2016 Category: Biochemistry Authors: Ryan G. Snodgrass, Shurong Huang, Dmitry Namgaladze, Ola Jandeli, Tiffany Shao, Spandana Sama, Bernhard Brüne, Daniel H. Hwang Source Type: research