Étude prospective comparant l’efficacité de la vitrification ovocytaire en système fermé ou en système ouvert

Conclusion Dans les limites de notre protocole, la vitrification des ovocytes en SO reste actuellement le système le plus efficace. L’utilisation d’azote stérile pour la vitrification et le stockage en vapeur d’azote pourraient permettre de minimiser le risque, par ailleurs hypothétique, de contaminations biologiques et chimiques. Objectives Oocyte vitrification using an open device is thought to be a source of microbiological and chemical contaminations that can be avoided using a closed device. The principal purpose of this study was to compare the two vitrification protocols: closed and open system. The secondary aim was to study the effects of the storage in the vapor phase of nitrogen (VPN) on oocytes vitrified using an open system and to compare it to those of a storage in liquid nitrogen (LN). Methods Forty-four patients have been included in our study between November 2014 and May 2015. Two hundred and fourteen oocytes have been vitrified at germinal vesicle (GV), metaphase I (0PB) and metaphase II (1PB) stages. We vitrified 96 oocytes (59 GV/37 0PB) using a closed vitrification device and 118 oocytes (57 GV/31 0PB/30 1PB) using an open device. The vitrified oocytes were then stored either in LN or in VPN. The main outcome measures were the survival rate after warming (SR), meiosis resumption rate (MRR) and maturation rate (MR). Results The global post-thaw SR was significantly higher for oocytes vitrified using an open system (93.2%) compare...
Source: Gynecologie Obstetrique and Fertilite - Category: OBGYN Source Type: research