In vitro study of aqueous leaf extract of Chenopodium album for inhibition of calcium oxalate and brushite crystallization

Publication date: Available online 2 March 2016 Source:Egyptian Journal of Basic and Applied Sciences Author(s): Deepti Sharma, Yadu Nandan Dey, Indu Sikarwar, Richa Sijoria, Manish M. Wanjari, Ankush D. Jadhav The leaves of Chenopodium album Linn. are traditionally used for treatment of kidney diseases and urinary stones. The present work investigated the effect of aqueous extract of leaves of C. album (CAAE) on in-vitro crystallization of CaOx and brushite crystals. Crystallization was studied by using nucleation and aggregation assay of calcium oxalate (CaOx) crystals and growth assay of calcium oxalate monohydrate and brushite crystals. The effects of CAAE and cystone on slope of nucleation and aggregation as well as growth of calcium oxalate crystallization were evaluated spectrophotometrically. The densities of the formed crystals were compared under microscope. The effects of CAAE and citric acid on growth of brushite crystals were carried out by using single diffusion gel growth technique, and the parameters evaluated were length, morphology and average size of the deposited crystals. CAAE significantly inhibited the slope of nucleation and aggregation of CaOx crystallization, and decreased the crystal density. It also inhibited the growth and caused the dissolution of brushite crystals. The standard drug cystone or citric acid also exhibited similar effects. The study reveals that the leaves of C. album were found effective in the prevention of th...
Source: Egyptian Journal of Basic and Applied Sciences - Category: Science Source Type: research