A Cyan Fluorescent Reporter Expressed from the Chloroplast Genome of Marchantia polymorpha

We report the design of a codon-optimized gfp varian, mturq2cp, which allowed successful expression of a cyan fluorescent protein under control of the tobacco psbA promoter from the chloroplast genome of M. polymorpha. We demonstrate the utility of mturq2cp in (i) early screening for transplastomic events following biolistic transformation of M. polymorpha spores; (ii) visualization of stromules as elements of plastid structure in Marchantia; and (iii) quantitative microscopy for the analysis of promoter activity.

Source: Plant and Cell Physiology - February 23, 2016 Category: Cytology Authors: Boehm, C. R., Ueda, M., Nishimura, Y., Shikanai, T., Haseloff, J. Tags: Special Focus Issue - Regular Papers Source Type: research