Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics

Publication date: Available online 22 February 2016 Source:EuPA Open Proteomics Author(s): Alice Di Pierro, Heather Bondi, Chiara Monti, Luisa Pieroni, Enrico Cilio, Andrea Urbani, Tiziana Alberio, Mauro Fasanoab, Maurizio Ronci Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research