Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

Conclusions The NE095 strain was successfully detected by all participants, with the high concentration indicating a potential target concentration for a reference material. Significance and impact of the study The engineered yeast has potential to support measurement assurance for the analytical process of qPCR, encompassing the method, equipment, and operator, to increase confidence in results and better inform decision-making in areas of applied microbiology. This material can also support process assessment for other DNA-based detection technologies.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research