An Alternative Thiol-Reactive Dye to Analyze Ligand Interactions with the Chemokine Receptor CXCR2 Using a New Thermal Shift Assay Format

This study showed that the new DSF assay assessed reliably the stability of CXCR2 in a 384-well format. The analysis of 14 ligands with a potency range over 4 log units demonstrated the detection/characterization of LMW ligands binding to the membrane protein target. Furthermore, DSF results cross-validated with the label-free differential static light scattering (DSLS) thermal denaturation method. These results underline the potential of the BODIPY assay format as a general tool to investigate membrane proteins and their interaction partners.

Source: Journal of Biomolecular Screening - February 19, 2016 Category: Molecular Biology Authors: Bergsdorf, C., Fiez-Vandal, C., Sykes, D. A., Bernet, P., Aussenac, S., Charlton, S. J., Schopfer, U., Ottl, J., Duckely, M. Tags: Original Research Source Type: research