Cyclin D1 level, ploidy status, and S-phase fraction in colorectal cancer patients

Background: Colorectal carcinoma (CRC) is a common neoplasm representing the third most common cancer worldwide. In Egypt, it constitutes 5% of the total estimated cancers. Cyclin D1 is overexpressed in CRC. The prognostic value of cyclin D1 together with ploidy status and S-phase fraction (SPF) is still conflicting. Aim: The aim of this study was to assess the prognostic value of cyclin D1, ploidy status, and SPF in CRC patients and evaluate its impact by correlating with histopathological characters. Materials and methods: A total of 50 specimens of CRC were collected from National Cancer Institute, Cairo University. The upregulation of cyclin D1 was assessed by means of immunohistochemistry using tissue microarray assay. The ploidy status and SPF were also determined using flow cytometry and compared with cyclin D1 expression as prognostic factors for CRC. Results: Of the 50 cases examined, cyclin D1 was overexpressed in 78% of cases. On the other hand 24.4% of the cases were aneuploid with high SPF. A significant relation was detected between high tumor stages and glandular adenocarcinoma type with cyclin D1 overexpression as well as between SPF and ploidy status.
Source: Egyptian Journal of Pathology - Category: Pathology Tags: Original Articles Source Type: research