Développement embryonnaire dans 2 milieux de culture globale : étude prospective autocontrôlée sur 2059 ovocytes

Conclusion La culture embryonnaire dans le milieu global Fert/Sage One Step® vs le milieu global Continuous Single Culture® ne montre pas de différence dans le développement embryonnaire, ni dans le taux d’embryons utiles, transférés ou congelés. Objective The aim of this study was to compare embryo development cultured in two single-step media commercially available: Fert/Sage One Step® (Origio) and Continuous Single Culture® (CSC) (Irvine Scientific). Methods A prospective auto-controlled study of sibling oocytes from women undergoing conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) was performed in our center from February to June 2015. After fertilization, for every patient, half of oocytes were cultured in the single-step Fert/Sage One Step® (serie SAGE) and the other half in the single-step CSC®(serie CSC). Fertilization and embryo morphology rates were assessed by day 1 to day 5–6 if needed. Embryo presenting<20% of fragmentation and 4 cells at day 2, 8 cells at day 3 were qualified as “top quality”. Embryo with<20% of fragmentation and 3–5 cells at day 2, 6–10 cells at day 3 were qualified as “good quality”. Blastocyst B3, B4, B5 with A or B inner cell mass and A or B trophectoderm were qualified as “good quality”. Transferred or frozen embryos were qualified as useful embryos. Results Sixty-two attempts of IVF and 133 of ICSI were analyzed, corresponding to 2059 inseminated or micr...
Source: Gynecologie Obstetrique and Fertilite - Category: OBGYN Source Type: research