Antibody-mediated inhibition of Nogo-A signaling promotes neurite growth in PC-12 cells

In this study, we developed and validated a novel cell-based approach to facilitate the biological quantification of a Nogo-A antibody using PC-12 cells as an in vitro neuronal cell model. Changes in the mRNA levels of the neuronal differentiation markers, growth-associated protein 43 and neurofilament light-polypeptide, suggest that activation of the Nogo-A pathway suppresses axonal growth and dendrite formation in the tested cell line. We found that application of anti-Nogo-A monoclonal antibody can significantly enhance the neuronal maturity of PC-12 cells by blocking the Nogo-A inhibitory effects, providing enhanced effects on neural maturity at the molecular level. No adverse effects were observed on cell viability.

Source: Journal of Tissue Engineering - February 16, 2016 Category: Biotechnology Authors: Yazdi, I. K., Taghipour, N., Hmaidan, S., Palomba, R., Scaria, S., Munoz, A., Boone, T. B., Tasciotti, E. Tags: Original Article Source Type: research