Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule

The engineering of green fluorescent protein (GFP) fusion constructs in order to visibly tag a protein of interest has become a commonly used cell biology technique. Although caveats to this approach are obvio...

Source: Journal of Negative Results in BioMedicine - September 10, 2004 Category: Research Authors: Dennis A Hanson and Steven F Ziegler Source Type: research