Multi‐color FISH facilitates analysis of cell‐type diversification and developmental gene regulation in the Parasteatoda spider embryo

This study proposes that mFISH is a powerful tool for the cell‐level analysis of gene regulation and quantification in the spider model.

We established a method for multi‐color fluorescence in situ hybridization (mFISH) in the spider embryo. This mFISH technique allowed quadruple staining using four kinds of labels for RNA probes, and it was used for the analysis of overlapping gene expression in the cell level, the relative quantification of gene expression, and combined examination with embryonic RNA interference.

Source: Development, Growth and Differentiation - January 1, 2016 Category: Research Authors: Yasuko Akiyama‐Oda, Hiroki Oda Tags: Original Article Source Type: research