Enzyme-catalyzed asymmetric construction of chiral tertiary alcohols via aldol reaction using proteinase

Publication date: Available online 6 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Rui Li, Zhi-Lin Li, Hai-Yan Zhou, Yan-Hong He, Zhi Guana A new enzyme-catalyzed asymmetric construction of chiral tertiary alcohols via asymmetric aldol reactions between β,γ-unsaturated α-keto esters and ketones was reported. Proteinase from Aspergillus melleus (AMP) was used as a sustainable biocatalyst. The best results can be obtained with yields of up to 90%, diastereoselectives of up to 93:7 dr, and enantioselectivities of up to 70% ee. This work not only expands the application of enzymatic promiscuity, but also provides more examples for constructing chiral tertiary alcohols. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research