Study on Lipozyme TL IM-catalyzed esterification of oleic acid and glycerol for 1,3-diolein preparation

Publication date: Available online 6 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Zitian Wang, Wei Du, Lingmei Dai, Dehua Liu It is well recognized that oils with a high 1,3-diacylglycerol content has the advantage of suppressing the accumulation of visceral fat and preventing the increase of body weight, and the synthesis of 1,3-diacylglycerol has attracted considerable attention. It is important to explore various methods for 1,3-diacylglycerol synthesis. In this work, the potential of using lipase Lipozyme TL IM-mediated esterification of oleic acid with glycerol for 1,3-diolein preparation was explored. The effect of solvent, oleic acid feeding strategy, online water removal, water activity, temperature, molar ratio of oleic acid to glycerol and enzyme dosage on the diolein yield and 1,3-positional selectivity of the lipase was investigated systematically. It was found that solvent, water activity, temperature, substrate molar ratio and enzyme dosage had varied influence on diolein yield as well as the positional selectivity of the enzyme. With the increasing water activity and solvent’s log P, diolein yield and the positional selectivity increased to a maximum value at a certain point, then had a decrease. It seems that in the process with multiple reaction involved, reactions trend to generate the products with log P closer to the system log P. In addition, with the water activity being closer to its optimum water activity, t...

Source: Journal of Molecular Catalysis B: Enzymatic - February 7, 2016 Category: Biochemistry Source Type: research