Enhancement of Glucose Isomerase activity by immobilizing on silica/chitosan hybrid microspheres

In this study, GI was immobilized on silica/chitosan hybrid microspheres via simple process through in situ encapsulation. Enhanced rate of reaction was observed when the conversion of glucose to fructose was completed in 10 mins catalyzed by immobilized GI because most GI was located on the shell of the support. Moreover, it was found that immobilized GI exhibited better pH, temperature, ions, storage and operation stability when compared to free GI. The relative enzyme activity was found to be above 90% with a wide pH range of 5.8∼8.0, temperature range of 40∼80°C, storage range of 3 months and an increase in operation range of>15 times. Therefore, immobilized GI supported by silica/chitosan hybrid microspheres is an ideal candidate for biocatalysis. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - February 5, 2016 Category: Biochemistry Source Type: research