Isolation of T Cells Using Rosetting Procedures.

Isolation of T Cells Using Rosetting Procedures. Curr Protoc Immunol. 2016;112:7.2.1-7.2.5 Authors: Kanof ME Abstract This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2-aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells. It should be noted that use of the rosetting technique to obtain purified T cells or purified non-T cells by negative selection has largely been superceded by other techniques such as panning and immunomagnetic beads. © 2016 by John Wiley & Sons, Inc. PMID: 26836511 [PubMed - as supplied by publisher]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research