Lentiviral expression of GAD67 and CCK promoter‐driven opsins to target interneurons in vitro and in vivo

ConclusionsLV‐CCK‐NpHR2.0‐EYFP may be useful to target CCK+ interneurons in culture. GAD67/CCK‐driven lentiviral constructs are expressed in vivo, but expression is not specific for interneurons. Overall, expression levels are low compared to opsins driven by pyramidal cell promoters. Better understanding of GAD67 and CCK promoter structure or alternative techniques are required to reliably target opsins to interneurons using viral vectors. This article is protected by copyright. All rights reserved.

http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1002%2Fjgm.2873

Source: The Journal of Gene Medicine - January 29, 2016 Category: Genetics & Stem Cells Authors: Laura Mantoan Ritter, Douglas C. Macdonald, Georg Ritter, David Escors, Francesca Chiara, Anna Cariboni, Stephanie Schorge, Dimitri M. Kullmann, Mary Collins Tags: Research Article Source Type: research