Structural characteristics of polysaccharides extracted from Cladophora glomerata Kützing affecting nitric oxide releasing capacity of,RAW 264.7 cells

Publication date: Available online 28 January 2016 Source:Bioactive Carbohydrates and Dietary Fibre Author(s): Utoomporn Surayot, Ju Hun Lee, WooJung Park, SangGuan You The effect of molecular structures of sulfated polysaccharide (NF1) isolated from Cladophora glomerata Kützing on the NO releasing capacity was investigated. It was possible to obtain the NF1 derivatives having various amounts of proteins (2.55-8.50%) and sulfates (6.88-13.1%) as well as different M w (141-610 × 103 g/mol), which enabled to investigate the effects of various proteins, sulfates and M w of NF1 on the NO releasing capacity from RAW 264.7 cells. The activity was lost after desulfation of the polysaccharides and moreover slightly increased NO releasing capacity after oversulfation of NF1 polysaccharides with the degree of sulfation (DS, 3.70). Suggesting that the sulfate groups were essential to activate NF1 polysaccharides are key factors to regulate the immunomodulatory activities. Graphical abstract
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research