Effect of heparin, caffeine and calcium ionophore A 23187 on in vitro induction of the acrosome reaction of fresh ram spermatozoa

Conclusion The best concentration of heparin, caffeine and ionophore A23187 are 75 μg/mL, 1 mg/mL, 1.55 mM/mL for 3, 1, 4 hours incubation respectively and can be used for in vitro fertilization in sheep.
Source: Asian Pacific Journal of Reproduction - Category: Reproduction Medicine Source Type: research