Excessive Cellular Proliferation Negatively Impacts Reprogramming Efficiency of Human Fibroblasts

In this study, human fibroblasts cultured in media containing different concentrations of growth factors such as insulin and insulin-like growth factor-1 exhibited variable abilities to proliferate, with consequences on pluripotency. This occurred in part because of changes in the expression of proteins involved in the growth factor signaling pathway, glycolysis, and oxidative phosphorylation. These findings have implications for efficient reprogramming of human cells.

Source: Stem Cells Translational Medicine - September 17, 2015 Category: Stem Cells Authors: Gupta, M. K., Teo, A. K. K., Rao, T. N., Bhatt, S., Kleinridders, A., Shirakawa, J., Takatani, T., Hu, J., De Jesus, D. F., Windmueller, R., Wagers, A. J., Kulkarni, R. N. Tags: Pluripotent Stem Cells, Pluripotent Stem Cells Source Type: research