MiR-30b regulates migration and invasion of human colorectal cancer via SIX1

Colorectal cancer (CRC) is one of the most malignant tumors in both developing and developed countries. It is estimated that 60% of CRC patients bear liver metastasis. Here, we revealed that miR-30b is an important regulator in human CRC liver metastasismigration and invasion which are vital steps in CRC liver metastasis of human CRCs. MiR-30b was significantly downregulated in primary CRC specimens compared with normal tissues. Furthermore, miR-30b was much lower in liver metastasis (LM) tissues than in CRCs. We validated SIX1, a member of the six-homeodomain family of transcription factorsand an EMT (epithelial-to-mesenchymal transition) promoting gene, as the direct target of miR-30b. Forced expression of miR-30b inhibited CRC cell proliferation, migration and invasion in vitro via its target gene SIX1. Furthermore, an inverse correlation between expression of SIX1 and miR-30b has been observed both in primary CRC specimens and LM. Taken together, miR-30b plays an important role in mediating EMT and metastatic related behavior in CRC. MiR-30b may serve as a potential diagnostic marker and therapeutic target for CRC patients in the future.
Source: BJ Cell - Category: Biochemistry Authors: Tags: BJ Cell Source Type: research